We offer hands-on training courses on iPSC culture methods, an example of our 2-day training program is below.
9:00AM-9:15AM Introduction (Lurie conference room).
9:15AM-10:15AM Lecture –hPSC Introduction: Maintenance and Culture
10:30AM-12:00PM Lab Session –hPSC Morphology & Passaging (Lurie 10-234)
hPSC Morphology: Culture observation; Identification; Manually clean up differentiation;
Coating: Matrigel vs. Vitronectin XF
12:00PM-1:00PM Lunch Break
1:00PM- 3:00PM Lab Session – Passaging hPSCs in mTeSR (Lurie 10-234)
Passaging 1: Clump vs. Single-cell, with manual selection (GCDR)
Passaging 2: Clump, w/o manual selection (ReLeSR)
3:15PM-4:00PM Lab Session –Thawing hPSCs (Lurie 10-234)
Clump vs. Single-Cell
4:00PM-4:30PM Questions and Answers
Day 2 (Friday, October 12th)
9:00AM-9:45AM Lecture –hPSC Directed Differentiation (Lurie conference room)
(Neural, Hematopoietic, Cardiomyocytes and more).
10:00AM-10:30AM Lab Session – Day 1 Lab Review (Lurie 10-234)
Examining passaged and recovered hPSCs from Day 1 sessions.
10:30AM-11:30AM Lab Session – Cryopreservation of hPSCs (Lurie 10-234)
11:30AM-12:00PM Lecture – Introduction to iPSC Core Services (Lurie 10-234)
12:00AM-12:15PM Questions, Feedback Survey