We offer hands-on training courses on iPSC culture methods, an example of our 2-day training program is below.

Day 1

9:00AM-9:15AM     Introduction (Lurie conference room).

9:15AM-10:15AM   Lecture –hPSC Introduction:  Maintenance and Culture     

10:15AM-10:30AM  Break

10:30AM-12:00PM  Lab Session –hPSC Morphology & Passaging (Lurie 10-234)

hPSC Morphology:  Culture observation; Identification; Manually clean up differentiation;

Coating:  Matrigel vs. Vitronectin XF

12:00PM-1:00PM     Lunch Break

1:00PM- 3:00PM      Lab Session – Passaging hPSCs in mTeSR (Lurie 10-234)

Passaging 1: Clump vs. Single-cell, with manual selection (GCDR)

Passaging 2: Clump, w/o manual selection (ReLeSR)

3:00PM-3:15AM       Break

3:15PM-4:00PM       Lab Session –Thawing hPSCs (Lurie 10-234)

Clump vs. Single-Cell

4:00PM-4:30PM       Questions and Answers

Day 2 (Friday, October 12th)

9:00AM-9:45AM       Lecture –hPSC Directed Differentiation (Lurie conference room)

(Neural, Hematopoietic, Cardiomyocytes and more).

9:45AM-10:00AM     Break

10:00AM-10:30AM  Lab Session – Day 1 Lab Review (Lurie 10-234)                                            

Examining passaged and recovered hPSCs from Day 1 sessions.

10:30AM-11:30AM Lab Session – Cryopreservation of hPSCs (Lurie 10-234)

11:30AM-12:00PM  Lecture – Introduction to iPSC Core Services (Lurie 10-234)

12:00AM-12:15PM Questions, Feedback Survey