Single-Cell Genomics Facility
The Single-Cell Genomics Core Facility is a shared resource on the Evanston campus of Northwestern University, run by a full-time PhD-level core manager and faculty director. The facility provides services such as flow cytometry, FACS sorting, cell encapsulation, library generation, and spatial transcriptomics. The facility occupies a 1,000 square foot newly renovated, fully functional wet-lab space, including a clean lab for PCR and library preparation. The lab is equipped with a new BD FACS Discover S8 spectral imaging cell sorter and a BD Fortessa flow analyzer, which are run in conjunction with the staff of the RHLCCC Flow Cytometry Core Facility (based at the Feinberg School of Medicine). The facility also houses a 10x Genomics Chromium iX controller for single cell encapsulation, an Agilent 2100 Bioanalyzer, an Applied Biosystems QuantStudio 3 qPCR machine, 2 Applied Biosystems VertiPro Thermal Cyclers, a Vizgen MERSCOPE for multiplexed in situ hybridization-based spatial transcriptomics, a Revvity K2 Cell Counter, an Epredia NX70 cryostat, and a Zeiss Axiovert fluorescence microscope.
