Peng, A., King, M.C., Ross, M.O., Hoffman, B.M., King, H.H. The Role of Co=ZSM-5 Catalysts in Aerobic Oxidation of Ethylbenzene, Topics in Catalysis, 2020
Offenbacher, A.R., Sharma, A., Doan, P.E., Klinman, J.P., Hoffman, B.M. The Soybean Lipoxygenase–Substrate Complex: Correlation between the Properties of Tunneling-Ready States and ENDOR-Detected Structures of Ground States, Biochemistry 2020 59 (7) 901-910
Seefeldt, Lance C., Yang, Zhi-Yong, Lukoyanov, Dmitriy A., Harris, Derek F., Dean, Dennis R., Raugei, Simone, Hoffman, Brian M. Reduction of Substrates by Nitrogenases, Chem Rev. 2020 120 (12) 5082-5106
Ross, Matthew O., Fisher, Oriana S., Morgado, Marcus N., Krzyaniak, Matthew D., Wasliewski, Michael R., Vila, Alejandro A., Hoffman, Brian M., Rosenzweig, Amy C. Formation and electronic structure of an atypical CuA site, Journal of the American Chemical Society, 2019, 141 (11), 4678–4686
Ross, Matthew O., MacMillan, Fraser, Wang, Jingzhou, Nisthal, Alex, Lawton, Thomas J., Olafson, Barry D., Mayo, Stephen L., Rosenzweig, Amy C., Hoffman, Brian M. (2019) Particulate methane monooxygenase contains only mononuclear copper centers, Science, 2019, 364, (6440), 566-570
Hoeke, V., Tociu, L., Case, D. A., Seefeldt, L. C., Raugei, S., Hoffman, B. M. High resolution ENDOR spectroscopy combined with quantum chemical calculations reveals the structure of the nitrogenase Janus intermediate E4(4H), J. Am. Chem. Soc. 2019 141, 11984-11996
Yang, H., McDaniel, E. C., Impano, S., Byer, A. S., Jodts, R. J., Yokoyama, K., Broderick, W. E., Broderick, J. B., Hoffman, B. M. The Elusive 5′-Deoxyadenosyl Radical: Captured and Characterized by Electron Paramagnetic Resonance and Electron Nuclear Double Resonance Spectroscopies, J. Am. Chem. Soc. 2019, 141, 12139-12146
Harris, D. F., Lukoyanov, D. A., Kallas, H., Trncik, C., Yang, Z.-Y., Compton, P., Kelleher, N., Einsle, O., Dean, D. R., Hoffman, B. M., Seefeldt, L. C. Mo-, V-, and Fe-Nitrogenases Use a Universal Eight-Electron Reductive-Elimination Mechanism to Achieve N2 Reduction, Biochemistry 2019, 58, 3293-3301
Van Stappen, C., Davydov,R., Yang, Z.-Y., Fan, R., Guo, Y., Bill, E., Seefeldt, S. C., Hoffman, B. M., DeBeer, S. A spectroscopic description of the E1 state of Mo Nitrogenase based on Mo and Fe X-ray absorption and Mossbauer studies, Inorg. Chem. 2019, 58, 12365-12376
Lukoyanov, D. A., Krzyaniak, Matthew D., Dean, Dennis R., Wasielewski, Michael R., Seefeldt, Lance C., Hoffman, Brian M. Time-Resolved EPR Study of H2 Reductive Elimination from the Photoexcited Nitrogenase Janus E4(4H) Intermediate, J. Phys. Chem. B 2019, 123 (41), 8823-8828
Kenney, Grace E.; Dassama, Laura M. K.; Manesis, Anastasia C.; Ross, Matthew O.; Chen, Siyu; Hoffman, Brian M.; Rosenzweig, Amy C. MbnH is a diheme MauG-like protein associated with microbial copper homeostasis, JBC 2019, 294, 16141-16151
Fisher, Oriana S., Sendzik, Madison R., Ross, Matthew O., Lawton, Thomas J., Hoffman, Brian M., Rosenzweig, Amy C. PCu A C domains from methane-oxidizing bacteria use a histidine brace to bind copper, JBC, 2019, 294, 16351-16363
Yang, Hao; Impano, Stella, Shepard, Eric M., James, Christopher D., Broderick, William E., Broderick, Joan B., Hoffman, Brian M. Photoinduced Electron Transfer in a Radical SAM Enzyme Generates an S-Adenosylmethionine Derived Methyl Radical, JACS 2019 141, 16117-16124
Davydov, Roman M., Jennings, Gareth, Hoffman, Brian M., Podust, Larissa M. Short-lived neutral FMN and FAD semiquinones are transient intermediates in cryo-reduced yeast NADPH-cytochrome P450 reductase, Arch. Biochem. Biophys. 2019 623, 108080
Tsednee, Munkhtsetseg, Castruita, Madeli, Salome, Patrice A., Sharma, Ajay, Lewis, Brianne E., Schmollinger, Stefan R., Strenkert, Daniela, Holbrook, Kristen, Otegui, Marisa S., Khatua, Kaustav, Das, Sayani, Datta, Akona, Chen, Si, Ramon, Christina, Ralle, Martina, Weber, Peter K., Stemmler, Timothy L., Pett-Ridge, Jennifer, Hoffman, Brian M., Merchant, Sabeeha S. Manganese co-localizes with calcium and phosphorous in Chlamydomonas acidocalcisomes and is mobilized in manganese-deficient conditions, J. Biol. Chem. 2019 294 (46), 17626-17641
Harris, D. F., Lukoyanov, D. A., Shaw, S., Compton, P., Tokmina-Lukaszewska, M., Bothner, B., Kelleher, N., Dean, D. R., Hoffman, B. M., and Seefeldt, L. C. The Mechanism of N2 Reduction Catalyzed by Fe-Nitrogenase Involves Reductive Elimination of H2, Biochemistry 2018, 57, 701-710
Purohit, R., Ross, M. O., Bateluc, S., Kusowskic, A., Stemmler, T. L., Hoffman, B. M., and Rosenzweig, A. C. Cu+-Specific CopB Transporter: Revising P1b-type ATPase classification, PNAS 2018, 115, 2108-2113
Dong, M., Kathiresan, V., Fenwick, M. K., Torelli, A. T., Zhang, Y., Caranto, J. D., Dzikovski, B., Sharma, A., Lancaster, K. M., Freed, J. H., Ealick, S. E., Hoffman, B. M., and Lin, H. Organometallic and Radical Intermediates Reveal Mechanism of Diphthamide Biosynthesis, Science 2018, 359, 1247-1250
Chen, J. G., Crooks, R. M., Seefeldt, L. C., Bren, K. L., Bullock, R. M., Darensbourg, M. Y., Holland, P. L., Hoffman, B. M., Janik, M. J., Jones, A. K., Kanatzidis, M. G., King, P., Lancaster, K. M., Lymar, S. V., Pfromm, P., Schneider, W. F., and Schrock, R. R. Beyond Fossil Fuel–Driven Nitrogen Transformations, Science 2018, 360, 1-7, eaar6611
Lukoyanov, D. A., Khadka, N., Yang, Z.-Y., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. Hydride Conformers of the Nitrogenase FeMo-cofactor Two-Electron Reduced State E2(2H), Assigned Using Cryogenic Intra Electron Paramagnetic Resonance Cavity Photolysis, Inorg. Chem. 2018, 57, 6847-6852
Byer, A. S., Yang, H., McDaniel, E. C., Kathiresan, V., Impano, S., Pagnier, A., Watts, H., Denler, C., Vagstad, A., Piel, J., Duschene, K. S., Shepard, E. M., Shields, T. P., Scott, L. G., Lilla, E. A., Yokoyama, K., Broderick, W. E., Hoffman, B. M., and Broderick, J. B. A New Paradigm for Radical S‑Adenosyl‑L‑Methionine Reactions: The Organometallic Intermediate Ω Is Central to Catalysis, J. Am. Chem. Soc. 2018, 140, 8634-8638
Gallagher, A. T., Lee, J. Y., Kathiresan, V., Anderson, J. S., Hoffman, B. M., and Harris, T. D. A Structurally-Characterized Peroxomanganese(IV) Porphyrin from Reversible O2 Binding within a Metal–Organic Framework, Chem. Sci. 2018, 9, 1596-1603
Seefeldt, L. C., Hoffman, B. M., Peters, J. W., Raugei, S., Beratan, D. N., Antony, E., and Dean, D. R. (2018) Energy Transduction in Nitrogenase, Acc. Chem. Res., Acc. Chem. Res., 2018, 51 (9), pp 2179–2186
Ro, Soo Y; Ross, Matthew O.; Deng, Yue Wen; Batelu, Sharon; Lawton, Thomas J.; Hurley, Joseph D.; Stemmler, Timothy L.; Hoffman, Brian M.; Rosenzweig, Amy C. From Micelles to Bicelles: Effect of the Membrane on Particulate Methane Monooxygenase Activity, J. Biol. Chem., 2018, 293, (27), pp. 10457-10465
Fisher, Oriana S.; Kenney, Grace E.; Ross, Matthew O.; Ro, Soo Y.; Lemma, Betelehem E.; Batelu, Sharon; Thomas, Paul M.; Sosnowski, Victoria C.; DeHart, Caroline J.; Kelleher, Neil L.; Stemmler, Timothy L.; Hoffman, Brian M.; Rosenzweig, Amy C. Characterization of a Long Overlooked Copper Protein from Methane and Ammonia Oxidizing Bacteria, Nature Communications 2018, 9 (1), 4276
Raugei, S., Seefeldt, L. C., and Hoffman, B. M. (2018) A Critical Computational Analysis Illuminates the Reductive-Elimination Mechanism That Activates Nitrogenase for N2 Reduction, PNAS, 2018,115 (45), E10521-E10530, DOI: 10.1073/pnas.1810211115
Broderick, W. E., Hoffman, B. M., and Broderick, J. B. (2018) Mechanism of Radical Initiation in the Radical SAM Superfamily, Acc. Chem. Res., 2018, 51, 2611−2619.
Yang, H., Rittle, J., Marts, A. R., Peters, J. C., and Hoffman, B. M. (2018) ENDOR Characterization of (N2)FeII(μ-H)2FeI(N2)-: A Spectroscopic Model for N2 Binding by the di-μ-hydrido Nitrogenase Janus Intermediate, Inorg. Chem. 2018, 57, 12323-12330
Harris, D. F., Yang, Z.-Y., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. (2018) Kinetic Understanding of N2 Reduction Versus H2 Evolution at the E4(4H) Janus State in the Three Nitrogenases, Biochemistry, 2018, 57 (39), 5706-5714.
DOI: 10.1021/acs.biochem.8b00784
Seefeldt, Lance C., Peters, John W., Beratan, David N., Bothner, Brian, Minteer, Shelley D., Raugei, Simone and Hoffman, Brian M. Control of Electron Transfer in Nitrogenase, Chemical Biology, 2018, 47, 54-59
McWilliams, S. F.; Bunting, P. C.; Kathiresan, V.; Mercado, B. Q.; Hoffman, B. M.; Long, J. R.; Holland, P. L. Isolation and Characterization of a High-Spin Mixed-Valent Iron Dinitrogen Complex, Chem. Commun. 2018, 54, 13339-13342
Dong, M., Horitani, M., Dzikovski, B., Freed, J. H., Ealick, S., Hoffman, B.M., and Lin, H. (2017) Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adensylmethionine Enzymes in Diphthamide Biosynthesis J. Am. Chem. Soc. 139, 5680-5683
Horitani, M., Offenbacher, A. R., Carr, C. A., Yu, T., Hoeke, V., Cutsail, G. E., III, Hammes-Schiffer, S., Klinman, J. P., and Hoffman, B. M. (2017) 13C ENDOR Spectroscopy of Lipoxygenase-Substrate Complexes Reveals the Structural Basis for C-H Activation by Tunneling, J. Am. Chem. Soc. 139, 1984-1997
Lukoyanov, D., Khadka, N., Dean, D. R., Raugei, S., Seefeldt, L. C., and Hoffman, B. M. (2017) Photoinduced Reductive Elimination of H2 from the Nitrogenase Dihydride (Janus) State Involves a FeMo-Cofactor-H2 Intermediate, Inorg. Chem. 56, 2233-2240
Smith, A. T., Ross, M. O., Hoffman, B. M., and Rosenzweig, A. C. (2017) Metal Selectivity of a Cd-, Co-, and Zn-Transporting P1B-type ATPase, Biochemistry 56, 85-95
Patil, B. S., Hessel, V., Seefeldt, L. C., Dean, D. R., Hoffman, B. M., Cook, B. J., and Murray, L. J. (2017) Nitrogen Fixation, In Ullmann’s Encyclopedia of Industrial Chemistry, pp 1-21, Wiley-VCH Verlag GmbH & Co. KGaA
Shisler, K. A., Hutcheson, R. U., Horitani, M., Duschene, K. S., Crain, A. V., Byer, A. S., Shepard, E. M., Rasmussen, A., Yang, J., Broderick, W. E., Vey, J. L., Drennan, C. L., Hoffman, B. M., and Broderick, J. B. (2017) Monovalent Cation Activation of the Radical SAM Enzyme Pyruvate Formate-Lyase Activating Enzyme, J. Am. Chem. Soc. 139, 11803-11813
Khadka, N., Milton, R. D., Shaw, S., Lukoyanov, D., Dean, D. R., Minteer, S. D., Raugei, S., Hoffman, B. M., and Seefeldt, L. C. (2017) The Mechanism of Nitrogenase H2 Formation by Metal-Hydride Protonation Probed by Mediated Electrocatalysis and H/D Isotope Effects, J. Am. Chem. Soc. 139, 13518-13524
Horitani, M., Grubel, K., McWilliams, S. F., Stubbert, B. D., Mercado, B. Q., Yu, Y., Gurubasavaraj, P. M., Lees, N. S., Holland, P. L., and Hoffman, B. M. (2017) ENDOR Characterization of an Iron–Alkene Complex Provides Insight into a Corresponding Organometallic Intermediate of Nitrogenase, Chem. Sci. 8, 5941-5948.
Kandela, I. K., McAuli, K. J., Cochran, L. E., Barrett, A. G. M., Hoffman, B. M., Mazar, A. P., and Trivedi, E. R. (2017) Discovery of the Antitumor Effects of a Porphyrazine Diol (Pz 285) in Mda-Mb-231 Breast Tumor Xenograft Models in Mice, ACS Med. Chem. Lett 8, 705-709
Sharma, A., Roemelt, M., Reithofer, M., Schrock, R. R., Hoffman, B. M., and Neese, F. (2017) EPR/ENDOR and Theoretical Study of the Jahn−Teller-Active [HIPTN3N]MovL Complexes (L = N−, NH), Inorg. Chem. 56, 6906-6919
Nandwana, V., Ryoo, S.-R., Kanthala, S., Kumar, A., Sharma, A., Castro, F. C., Li, Y., Hoffman, B. M., Limef, S., and Dravid, V. P. (2017) Engineered Ferritin Nanocages as Natural Contrast Agents in Magnetic Resonance Imaging, RSC Adv. 7, 34892-34900
Sharma, A., Gaidamakova, E. K., Grichenko, O., Matrosova, V. Y., Hoeke, V., Klimenkova, P., Conze, I. H., Volpe, R. P., Tkave, R., Gostincar, C., Gunde-Cimerman, N., DiRuggiero, J., Shuryak, I., Ozarowski, A., Hoffman, B. M., and Daly, M. J. (2017) Across the Tree of Life, Radiation Resistance Is Governed by Antioxidant Mn2+, Gauged by Paramagnetic Resonance, PNAS 114, E9253-E9260
Danyal, K., Shaw, S., Page, T. R., Duval, S., Horitani, M., Marts, A. R., Lukoyanov, D., Dean, D. R., Raugei, S., Hoffman, B. M., Seefeldt, L. C., and Antony, E. (2016) Negative Cooperativity in the Nitrogenase Fe Protein Electron Delivery Cycle, PNAS 113, E5783-E5791
Davydov, R., Fleischhacker, A. S., Bagai, I., Hoffman, B. M., and Ragsdale, S. W. (2016) Comparison of the Mechanisms of Heme Hydroxylation by Heme Oxygenases-1 and -2: Kinetic and Cryoreduction Studies, Biochemistry 55, 62-68
Davydov, R., Im, S., Shanmugam, M., Gunderson, W. A., Pearl, N. M., Hoffman, B. M., and Waskell, L. (2016) Role of the Proximal Cysteine Hydrogen Bonding Interaction in Cytochrome P450 2B4 Studied by Cryoreduction, Electron Paramagnetic Resonance, and Electron-Nuclear Double Resonance Spectroscopy, Biochemistry 55, 869-883
Davydov, R., Khadka, N., Yang, Z. Y., Fielding, A. J., Lukoyanov, D., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. (2016) Exploring Electron/Proton Transfer and Conformational Changes in the Nitrogenase MoFe Protein and FeMo-cofactor through Cryoreduction/EPR Measurements, Isr J Chem 56, 841-851
Dong, M., Horitani, M., Dzikovski, B., Pandelia, M. E., Krebs, C., Freed, J. H., Hoffman, B. M., and Lin, H. (2016) Organometallic Complex Formed by an Unconventional Radical S-Adenosylmethionine Enzyme, J. Am. Chem. Soc. 138, 9755-9758
Horitani, M., Shisler, K., Broderick, W. E., Hutcheson, R. U., Duschene, K. S., Marts, A. R., Hoffman, B. M., and Broderick, J. B. (2016) Radical SAM Catalysis Via an Organometallic Intermediate with an Fe-[5′-C]-Deoxyadenosyl Bond, Science 352, 822-825
Kenney, G. E., Goering, A. W., Ross, M. O., DeHart, C. J., Thomas, P. M., Hoffman, B. M., Kelleher, N. L., and Rosenzweig, A. C. (2016) Characterization of Methanobactin from Methylosinus sp. LW4, J. Am. Chem. Soc. 138, 11124-11127
Khadka, N., Dean, D. R., Smith, D., Hoffman, B. M., Raugei, S., and Seefeldt, L. C. (2016) CO2 Reduction Catalyzed by Nitrogenase: Pathways to Formate, Carbon Monoxide, and Methane, Inorg. Chem. 55, 8321-8330
Lukoyanov, D., Khadka, N., Yang, Z. Y., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. (2016) Reversible Photoinduced Reductive Elimination of H2 from the Nitrogenase Dihydride State, the E4(4H) Janus Intermediate, J. Am. Chem. Soc. 138, 1320-1327
Lukoyanov, D., Khadka, N., Yang, Z. Y., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. (2016) Reductive Elimination of H2 Activates Nitrogenase to Reduce the N≡N Triple Bond: Characterization of the E4(4H) Janus Intermediate in Wild-Type Enzyme, J. Am. Chem. Soc. 138, 10674-10683
Petrik, I. D., Davydov, R., Ross, M., Zhao, X., Hoffman, B., and Lu, Y. (2016) Spectroscopic and Crystallographic Evidence for the Role of a Water-Containing H-Bond Network in Oxidase Activity of an Engineered Myoglobin, J. Am. Chem. Soc. 138, 1134-1137
Shelby, M. L., Lestrange, P. J., Jackson, N. E., Haldrup, K., Mara, M. W., Stickrath, A. B., Zhu, D., Lemke, H. T., Chollet, M., Hoffman, B. M., Li, X., and Chen, L. X. (2016) Ultrafast Excited State Relaxation of a Metalloporphyrin Revealed by Femtosecond X-ray Absorption Spectroscopy, J. Am. Chem. Soc. 138, 8752-8764
Trana, E. N., Nocek, J. M., Woude, J. V., Span, I., Smith, S. M., Rosenzweig, A. C., and Hoffman, B. M. (2016) Charge-Disproportionation Symmetry Breaking Creates a Heterodimeric Myoglobin Complex with Enhanced Affinity and Rapid Intracomplex Electron Transfer, J. Am. Chem. Soc. 138, 12615-12628
Anderson, J. S., Cutsail, G. E., III, Rittle, J., Connor, B. A., Gunderson, W. A., Zhang, L., Hoffman, B. M., and Peters, J. C. (2015) Characterization of an FeN-NH2 Intermediate Relevant to Catalytic N2 Reduction to NH3, J. Am. Chem. Soc. 137, 7803-7809
Bagai, I., Sarangi, R., Fleischhacker, A. S., Sharma, A., Hoffman, B. M., Zuiderweg, E. R., and Ragsdale, S. W. (2015) Spectroscopic Studies Reveal That the Heme Regulatory Motifs of Heme Oxygenase-2 Are Dynamically Disordered and Exhibit Redox-Dependent Interaction with Heme, Biochemistry 54, 2693-2708
Cutsail, G. E.III, Telser, J., and Hoffman, B. M. (2015) Advanced Paramagnetic Resonance Spectroscopies of Iron-Sulfur Proteins: Electron Nuclear Double Resonance (ENDOR) and Electron Spin Echo Envelope Modulation (ESEEM), Biochim. Biophys. Acta 1853, 1370-1394
Davydov, R., Strushkevich, N., Smil, D., Yantsevich, A., Gilep, A., Usanov, S., and Hoffman, B. M. (2015) Evidence That Compound I Is the Active Species in Both the Hydroxylase and Lyase Steps by Which P450scc Converts Cholesterol to Pregnenolone: EPR/ENDOR/Cryoreduction/Annealing Studies, Biochemistry 54, 7089-7097
Doan, P. E., Shanmugam, M., Stubbe, J., and Hoffman, B. M. (2015) Composition and Structure of the Inorganic Core of Relaxed Intermediate X(Y122F) of Escherichia coli Ribonucleotide Reductase, J. Am. Chem. Soc. 137, 15558-15566
Fleischhacker, A. S., Sharma, A., Choi, M., Spencer, A. M., Bagai, I., Hoffman, B. M., and Ragsdale, S. W. (2015) The C-Terminal Heme Regulatory Motifs of Heme Oxygenase-2 Are Redox-Regulated Heme Binding Sites, Biochemistry 54, 2709-2718
Horitani, M., Byer, A. S., Shisler, K. A., Chandra, T., Broderick, J. B., and Hoffman, B. M. (2015) Why Nature Uses Radical SAM Enzymes So Widely: Electron Nuclear Double Resonance Studies of Lysine 2,3-Aminomutase Show the 5′-dAdo• “Free Radical” Is Never Free, J. Am. Chem. Soc. 137, 7111-7121
Lukoyanov, D., Yang, Z. Y., Khadka, N., Dean, D. R., Seefeldt, L. C., and Hoffman, B. M. (2015) Identification of a Key Catalytic Intermediate Demonstrates That Nitrogenase Is Activated by the Reversible Exchange of N2 for H2, J. Am. Chem. Soc. 137, 3610-3615
Page, T. R., and Hoffman, B. M. (2015) Control of Cyclic Photoinitiated Electron Transfer between Cytochrome c Peroxidase (W191F) and Cytochrome c by Formation of Dynamic Binary and Ternary Complexes, Biochemistry 54, 1188-1197