Non-covalent Functionalization of Graphene Graphene functionalization remains a key challenge in developing graphene-based bioelectronics, biomedical devices, and functional graphene nanoconstructs. By designing molecular tripods, we have introduced a noncovalent strategy to functionalize pristine graphene with excellent kinetic stability and intriguing diffusional surface dynamics. Our tripodal strategy preserves the continuity of graphene’s hexagonal carbon network while imparting functionality to graphene’s pristine surface for bioconjugation of enzymes, antibodies, fluorophores, and more. The tripodal strategy has expanded our graphene research into the interface between biology and graphene for studies of graphene biosensors, fuel cells, and graphene oxide biomaterials. The pyrene-based strategy also promises exciting opportunities in engineering graphene physics.