The Wasielewski Group is well-equipped to carry out synthetic chemistry procedures in 16 6-foot fume hoods with vacuum lines and a separate hood hosting an Expedite oligo nucleic acid synthesizer. Purified anhydrous solvents are easily accessible from the Glas-Contour column solvent purification system. Instrumentation rooms adjacent to the synthesis laboratories house benchtop equipment, such as a spin coater, analytical and preparative HPLC equipment (in both normal and reverse phase), a Shimadzu GC system, CH Instruments Model 660 and 750 single/dual channel electrochemical analysis systems. Air-sensitive chemistry can be carried out in two nitrogen glove boxes (MBraun); another nitrogen box is equipped with a vacuum thermal evaporator (Denton) for thin-film preparation.
Electron Paramagnetic Resonance (EPR) Laboratory
Electron Paramagnetic Resonance (EPR) Spectroscopy Lab.
Our lab features state-of-the-art EPR measurement capability at X-, Ka- and W-bands. We have three spectrometers capable of both steady state and time resolved EPR measurement. Some of our capabilities include: CW EPR and electron-nuclear double-resonance (ENDOR) spectroscopies; time-resolved CW EPR; pulsed EPR experiments such as electron-spin echo modulation (ESEEM), ENDOR; and multi-frequency experiments such as electron-electron double resonance (ELDOR) spectroscopy. For sample photo-excitation, the lab is equipped with two nanosecond Nd;YAG lasers whose output is each coupled to an optical parametric oscillaotor (OPO) to generate outputs at 355 nm and 416-650 nm.
The primary workhorse in our lab is a recently upgraded Bruker E-680X/W Pulse-EPR spectrometer. This spectrometer operates at X-band (9.5 GHz or around 350 mT) as well as at W-band (94 GHz or ~3.5 T) and includes an ENDOR unit which operates at both fields. At X-band a 1 kW traveling wave tube (TWT) amplifier is employed to generate high-power microwave pulses, at W-band solid state amplifiers give 2 W of microwave power. This spectrometer is outfitted with an arbitrary waveform generator (AWG) which provides complete control over the frequency, shape, phase, and amplitude of the microwave pulses.
In addition, we have a second Bruker E-580X spectrometer that can operate in CW and Pulse modes at X-band. This spectrometer is also equipped with an ENDOR unit. While this spectrometer does not have an AWG, it does have 8 user-configurable microwave pulse forming units which allows for a greater degree of control over the amplitude and phase of the pulses compared to a conventional 4-channel spectrometer.
We also have a recently upgraded home-built Ka-band (34 GHz, ~1.2 T) spectrometer. This spectrometer designed to operate with a high resolution 8 GSa/s 14-bit AWG, generating a waveform with up to 2 GHz of bandwidth, which is up-converted to Ka-band. The spectrometer can operate in both CW and Pulse modes and in pulse mode it has a maximum output of 10 W.
The Wasielewski group has a recently renovated state-of-the-art laser laboratory with 6 individual rooms with individual climate control. Each room is positively pressurized to reduce contamination and maintain tight control over temperature and relative humidity, with a nominal 1 °C / 2% RH tolerance over 8 hours and over 8 months. Each room houses a laser system with dedicated experiments.