Symposium 2012

Northwestern University’s Cellular and Molecular Basis of Disease Training Grant Presents:

Mouse Transgenics Mini Course

 

Monday May 7th 2012, Frances Searle 2407, Evanston

Wednesday May 9th 2012, Baldwin Auditorium, Chicago

 

Transgenic mice have become an invaluable tool for dissecting molecular mechanisms in complex biological systems. With the ever-increasing prevalence and sophistication of genetic technology, a fundamental knowledge of mouse genetics is increasingly important in biological research.

 

OPEN TO EVERYONE.

NO REGISTRATION REQUIRED.

LUNCH PROVIDED!

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Mini Course Schedule:


**NOTE: All topics listed may not be covered due to time restrictions.

Monday May 7th , 1:00-4:30pm

Evanston, Frances Searle 2407

1:00-1:15 Introduction

 

1:15         Tom Bozza

An introduction to mouse transgenesis.

-Basic gene structure

-Transgene design

-Transgene propagation

-BAC transgenes

-Integration effects and targeted transgenesis

-Tracking gene expression in mice—reporters, fusions, and indicators

 

2:45         Break

 

3:00         Alec Wang

An introduction to targeted mutagenesis, chromosome engineering, and insertional mutagenesis.

-Design of gene knockouts and knock-ins

-Advances in cloning techniques

-ES cell homologous recombination

-Screening and common breeding schemes

-Electronic resources for targeted mutations

-Insertional mutagenesis—gene traps, transposons, nucleases

-Chromosome engineering

 

Wednesday May 9th, 10:30-2:00pm

Chicago, Baldwin Auditorium

 

10:30        Introduction

 

10:45       Lynn Doglio

Generating genetically modified mice

-Pronuclear injection

-Gene targeting in ES cells

-Generation of chimeric mice

-Germline transmission

-Common issues with germline transmission

-Cryopreservation and in vitro embryo recovery

-Northwestern Transgenic services

 

11:45       Break for Lunch

 

12:00       Raj Awatramani

Conditional genetic approaches

-Site specific recombination systems (Cre/lox, Flp/Frt, Dre/Rox)

-Inducible recombination strategies

-Intersectional genetics

-Fate mapping

 

1:00         Christine DiDonato:

Phenotypic analysis of mouse models

-Colony maintenance

-Drug administration

-Effects of genetic background on phenotypes

-Outbred, inbred, consomic and congenic lines

-Disease/behavior models

-The Mouse Phenome database

-Resources at Northwestern (Behavioral Core)

 

Please email any questions to [email protected]

 

Co-Sponsored by: The Driskill Graduate Program

 

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Want to hear about the latest research in mouse genetics from renowned scientists? Check out the Mouse Genetics Group Symposium and Poster Competition happening May14th, 2012.