Northwestern University’s Cellular and Molecular Basis of Disease Training Grant Presents:
Mouse Transgenics Mini Course
Monday May 7th 2012, Frances Searle 2407, Evanston
Wednesday May 9th 2012, Baldwin Auditorium, Chicago
Transgenic mice have become an invaluable tool for dissecting molecular mechanisms in complex biological systems. With the ever-increasing prevalence and sophistication of genetic technology, a fundamental knowledge of mouse genetics is increasingly important in biological research.
OPEN TO EVERYONE.
NO REGISTRATION REQUIRED.
Mini Course Schedule:
**NOTE: All topics listed may not be covered due to time restrictions.
Monday May 7th , 1:00-4:30pm
Evanston, Frances Searle 2407
1:15 Tom Bozza
An introduction to mouse transgenesis.
-Basic gene structure
-Integration effects and targeted transgenesis
-Tracking gene expression in mice—reporters, fusions, and indicators
3:00 Alec Wang
An introduction to targeted mutagenesis, chromosome engineering, and insertional mutagenesis.
-Design of gene knockouts and knock-ins
-Advances in cloning techniques
-ES cell homologous recombination
-Screening and common breeding schemes
-Electronic resources for targeted mutations
-Insertional mutagenesis—gene traps, transposons, nucleases
Wednesday May 9th, 10:30-2:00pm
Chicago, Baldwin Auditorium
10:45 Lynn Doglio
Generating genetically modified mice
-Gene targeting in ES cells
-Generation of chimeric mice
-Common issues with germline transmission
-Cryopreservation and in vitro embryo recovery
-Northwestern Transgenic services
11:45 Break for Lunch
12:00 Raj Awatramani
Conditional genetic approaches
-Site specific recombination systems (Cre/lox, Flp/Frt, Dre/Rox)
-Inducible recombination strategies
1:00 Christine DiDonato:
Phenotypic analysis of mouse models
-Effects of genetic background on phenotypes
-Outbred, inbred, consomic and congenic lines
-The Mouse Phenome database
-Resources at Northwestern (Behavioral Core)
Please email any questions to [email protected]
Co-Sponsored by: The Driskill Graduate Program
Want to hear about the latest research in mouse genetics from renowned scientists? Check out the Mouse Genetics Group Symposium and Poster Competition happening May14th, 2012.